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Amirhossein Ghadiri

Amirhossein Ghadiri

Biotechnology
Department Of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran · Iran
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About

I am a Ph.D. researcher in Microbial Biotechnology with a strong passion for advancing microbial science toward innovative industrial and biomedical applications. My research focuses on the isolation, characterization, and biotechnological exploitation of microorganisms with significant potential for producing high-value biomolecules and industrially important metabolites.

My scientific expertise encompasses microbial isolation and cultivation, molecular identification, phylogenetic analysis, DNA extraction, PCR-based techniques, and microbial screening for functional traits. I have been actively involved in the discovery and characterization of exopolysaccharides -producing microorganisms, as well as the isolation and molecular identification of probiotic Lactobacillus species from dairy products.

My broader research interests include industrial microbiology, microbial enzymes, extremophilic microorganisms, archaeal biotechnology, microbial pigments, exopolysaccharides, natural antimicrobial compounds, and sustainable bioprocess development. I am particularly interested in discovering novel microbial resources and translating fundamental microbiological research into practical industrial technologies.

Currently, I am developing research projects focused on the production and recombinant expression of thermostable enzymes from archaeal microorganisms, microbial production of extracellular polysaccharides, agarose extraction technologies, marine microbial biotechnology, and the integration of nanotechnology with microbial systems for biomedical and industrial applications.

My long-term scientific vision is to establish an internationally recognized biotechnology research and innovation platform that bridges academic research with industrial commercialization. I strongly believe that interdisciplinary collaboration among microbiologists, biotechnologists, chemists, food scientists, materials scientists, and engineers is essential for addressing global challenges in health, food security, and sustainable manufacturing.

Beyond academic research, I am committed to translating scientific discoveries into commercially viable technologies through entrepreneurship and innovation. My goal is to establish a biotechnology company dedicated to developing microbial-based products for the pharmaceutical, food, environmental, and industrial sectors.

I welcome international collaborations in microbial biotechnology, industrial microbiology, synthetic biology, metabolic engineering, microbial enzyme technology, food biotechnology, and bioprocess engineering. My current objective is to develop innovative microbial technologies that can be translated from laboratory research into industrial and commercial applications while fostering multidisciplinary collaborations with researchers, academic institutions, and biotechnology industries worldwide.

Research keywords

FermentationSynthetic BiologyBioinformaticsMicrobial Biotechnology

Publications

4

Biofilm formation in carbapenem-resistant Acinetobacter baumannii: Investigating the prevalence of the pgaABCD locus in clinical isolates

F1000Research · 2025

Background Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important nosocomial pathogen with high biofilm-forming ability and multidrug resistance. This study aimed to investigate the prevalence of the pgaABCD locus in clinical CRAB isolates and its relationship with biofilm production and antimicrobial resistance. Methods A total of 150 non-duplicate clinical A. baumannii isolates were collected from healthcare facilities in Shahrekord, Iran. Identification was performed by PCR and biochemical tests. Antimicrobial susceptibility was determined using disk diffusion, and biofilm formation was assessed by the crystal violet method. PCR was used to detect pgaABCD genes. Hemolytic and proteolytic activities were evaluated on blood and skim milk agar. Statistical analyses included chi-square tests and heatmap clustering of resistance profiles. Results Results showed that all isolates were multidrug-resistant, showing the highest resistance to ciprofloxacin (100%), ceftazidime (90%), and cefepime (86.66%). Of these, 90 (60%) were CRAB, all forming biofilms, with 55.5% producing strong biofilms. Hemolytic activity occurred in 14% of isolates and was significantly associated with strong biofilm formation (p < 0.0001). Proteolytic activity was observed in all isolates without significant association. The pgaA and pgaD genes were detected in 100% of isolates, while pgaB and pgaC were found in 84.4% and 93.3%, respectively. Strong biofilm formation correlated significantly with pgaB (p < 0.0001) and pgaC (p = 0.0002). Heatmap analysis showed higher antibiotic resistance in strong biofilm producers. Conclusion Strong biofilm formation in CRAB is associated with pgaB/pgaC genes and hemolysin production, highlighting the importance of anti-biofilm strategies and molecular monitoring in clinical settings.

Prevalence, Antimicrobial Susceptibility, and Distribution of Virulence Genes Involved in Biofilm Formation in Multidrug-Resistant <i>Acinetobacter baumannii</i> Isolated from Shahrekord Medical Centers, Chaharmahal and Bakhtiari, Iran

Iranian Journal of Medical Microbiology · 2023

Prevalence, Antimicrobial Susceptibility, and Distribution of Virulence Genes Involved in Biofilm Formation in Multidrug-Resistant Acinetobacter baumannii Isolated from Shahrekord Medical Centers, Chaharmahal and Bakhtiari, Iran

A Review of Bioinformatics Studies on the Function of Structural and Nonstructural Proteins and the Level of Glycoprotein Inhibiting Heme Metabolism by SARS-CoV-2 Virus

Jundishapur Journal of Medical Sciences · 2022

Coronavirus disease 2019 (COVID-19) is an acute respiratory infection. Its virus called SARS-COV-2 which is an RNA virus with high homology to the bat coronavirus. In this review study, first the molecular and cellular characteristics and the proliferation and replication of SARS-COV-2 are investigated. Then, by reviewing bioinformatics studies regarding protected domain analysis, homology modeling, and molecular docking, the biological role of some specific SARS-COV-2 proteins are examined. The results showed that the open reading frame 8 (ORF8) and surface glycoprotein could bind to porphyrin. At the same time, ORF1ab, ORF10, and ORF3a can attack the heme part of hemoglobin to dissociate iron and form porphyrin. This attack reduces hemoglobin ability to carry oxygen and carbon dioxide. As a result, lung cells become severely inflamed due to their inability to exchange carbon dioxide and oxygen, which leads to large ground-glass opacities on CT scan images. Based on the bioinformatics results, chloroquine can prevent ORF1ab, ORF3a, and ORF10 from attacking hemoglobin to form porphyrin and avoid the binding of ORF8 and surface glycoprotein to porphyrin, which effectively relieves the symptoms of acute respiratory syndrome. In the current pandemic, bioinformatics studies are of great importance for preventing the spread of COVID-19, developing drugs and vaccines, and clinical practice.

A review of the epidemiology and clinical signs of SARS-COV-2

New Cellular and Molecular Biotechnology Journal · 2020

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