Phytoecdysteroids are known to exert a broad range of biological activities in mammals accounting for a general strengthening, adaptogenic, and anabolic activity. Our research group has recently reported the synthesis of various autoxidized bioactive metabolites of 20-hydroxyecdysone (20E) [1]. Time dependency of the oxidation was monitored by HPLC after neutralizing the pH, and the above-mentioned metabolites were also detected when biomimetic oxidation of 20E using the Fenton reaction was performed and the product mixture was monitored by HPLC after sample pre-purification by solid phase extraction on silica [2]. In contrast with HPLC, capillary electrophoresis (CE) does not require sample pre-treatment, and it is also less time and solvent consuming.
DOI: https://doi.org/10.1055/s-0037-1608260
Publish Year: 2017
