Abstract The aim of this study is to compare histopathologically the protective effect of melatonin and amifostine on radiation induced renal damage. 50 female albino rats were divided into five groups: control, radiotherapy alone, radiotherapy+amifostine, radiotherapy+melatonin, radiotherapy+amifostine+melatonin. Intraperitoneal amifostine (200 mg/kg) and intraperitoneal melatonin (10 mg/kg) was administered to 30 minutes before irradiation. Rats were irradiated with a single dose of 8 Gy on whole body. At the end of the follow-up period, percentage of damaged glomeruli was determined by counting damaged glomeruli of kidney cortex as segmental or total necrosis for each animal. The protective effect of each agent has been shown, and there is an advantage in favor of melatonin (p = 0.005). Although there is not an advantage of adding amifostine to melatonin (p = 0.243), there is statistically significant better protective effect in amifostine+melatonin group when compared with amifostine alone (p = 0.003). The protective effect of melatonin on radiation induced acute renal toxicity is histopathologically better than amifostine. Key words: Amifostine, melatonin, acute renal damage, ionizing radiation. Ozet Bu calismanin amaci, erken donemde iyonizer radyasyonun neden oldugu akut bobrek hasari uzerine melatonin ve amifostinin koruyucu etkisinin histopatolojik olarak karsilastirilmasidir. 50 disi albino rat kontrol, yalniz radyoterapi, radyoterapi+amifostin, radyoterapi+melatonin ve radyoterapi+amifostin+melatonin olmak uzere bes gruba ayrildi. Intraperitoneal amifostin (200 mg/kg) ve intraperitoneal melatonin (10 mg/kg) radyoterapiden 30 dakika once uygulandi. Tek tek ratlarin tum vucutlarina 8 Gy eksternal radyopterapi uygulandi. Takip suresinin sonunda her bir hayvan icin segmental veya total nekroz gibi bobrek korteksi glomerul hasari sayilarak hasarli glomerul yuzdesi tespit edildi. Amifostin, melatonin ve amifostin+melatonin'in koruyucu etkisi istatistiksel olarak gosterilmekle birlikte melatonin lehine bir avantaj tespit edilmistir (p = 0.005). Melatonin’e amifostin eklenmesinin tek basina melatonine gore ustunlugu olmamasina karsin (p = 0.243) amifostine melatonin eklenmesinin tek basina amifostine gore istatistiksel ustunlugu gosterilmistir (p = 0.003). Sonuc olarak melatonin’in iyonizer radyasyonun neden oldugu akut bobrek hasari uzerine koruyucu etkisi amifostin’den histopatolojik olarak daha iyidir. Anahtar kelimeler: Amifostin, melatonin, akut renal hasar, iyonizer radyasyon.
Authors: Alaattin Özen, Ebru Taştekin, Suat Çakına, Şule Parlar, Nukhet Kurkcu, Necdet Süt, Cem Uzal
Publish Year: 2014
