Aims:The purpose of this study was to investigate the impact of various radiation doses on cellular elements using electron microscopy and to determine if non-irradiated cells in the same environment were also affected.Radiotherapy treatment is widely used for different types of cancers as it can prevent cell division and death through direct and indirect mechanisms.Methods: To conduct the study, standard strain Escherichia coli bacteria were reproduced in six Petri dishes, with one serving as the control.The other dishes were divided into two halves, and increasing radiation doses (2, 3, 6, 10, and 20 Gray) were administered to one side of each dish.Using a transmission electron microscope, the samples were examined, and changes in the cell elements were recorded.Results: The findings indicated that as the radiation doses increased, the negative effects on the bacteria were more pronounced.In the samples exposed to higher doses of 10 and 20 Gray, the bacteria's integrity was significantly impaired, resulting in a mud-like structure that made the evaluation of cell elements challenging.Furthermore, the non-irradiated area showed similar morphological deteriorations but at a lesser rate.Conclusion: This study demonstrated the effects of various radiation doses on Escherichia coli bacteria and the impact on non-irradiated cells in the same environment, as seen through transmission electron microscopy.
Authors: Semiha Pelin Kulaksız, Celal Çağlar, Huda Avvad, Shukran Alhmidi, Mohamad Ali Alhussein, Sheven Huseen, Alaattin Özen
DOI: https://doi.org/10.4274/tmsj.galenos.2023.2022-12-1
Publish Year: 2023
