<ns3:p>Introduction Traumatic ulcers are among the most prevalent injuries with a reported incidence of up to 95%. This study aims to evaluate Stem Cells from Human Exfoliated Deciduous Teeth Spent Medium (SHED-SM) nanoemulgel on the cytotoxicity of NIH-3T3 and buccal mucosal ulcer wounds. Materials and methods The cytotoxicity test of SHED-SM was analyzed on NIH-3T3 fibroblast cells using MTT. Thirty male Wistar rats, 2-3 months, were used as experimental subjects. Traumatic ulcer models were created by excising the right buccal mucosa with a 3 mm punch biopsy. Subjects were divided into 2 groups @15 rats for the treatment (SHED-SM) and control. The gel was applied on the surface once a day for 4 days. Evaluation was conducted on days 1, 3, 5, 7, and 14. Hematoxylin Eosin (HE) and immunohistochemical were stained to evaluate a number of neutrophils and the expression of COX-2, VEGF, and Col-1. Results The ANOVA results showed significant differences in the cytotoxicity test, the number of neutrophils, and the expression of COX-2, Col-1, and VEGF. The number of neutrophils and the expression of COX-2, Col-1, and VEGF were higher in the treatment group compared to the control. Conclusion SHED-SM demonstrates potential to accelerate the healing of traumatic buccal mucosal ulcers.</ns3:p>
DOI: https://doi.org/10.12688/f1000research.164339.1
Publish Year: 2025
