I am a Research Computing Technologist with a background in bioinformatics, molecular toxicology informatics, and high-performance computing (HPC). I hold a B.Sc. in Biotechnology and an M.Sc. in Pesticide Science with a focus on computational analysis of biological systems.
My work focuses on enabling large-scale scientific discovery by designing and operating accelerated research computing infrastructure. I support GPU-accelerated workloads, distributed computing environments, and scalable data analysis pipelines used in genomics, computational biology, and AI-driven biomedical research.
I have extensive experience working with Linux-based research systems, HPC clusters, SLURM scheduling, GPU computing, and containerized scientific workflows. My goal is to bridge the gap between advanced computing infrastructure and biological research to accelerate discoveries in life sciences and health-related fields.
I am open to interdisciplinary collaborations involving large-scale data analysis, computational biology, AI applications in life sciences, and research computing infrastructure.
The striped stem borer (SSB, <i>Chilo suppressalis Walker</i>) is a major pest of rice worldwide. Double-stranded RNAs (dsRNAs) targeting essential genes can trigger a lethal RNA interference (RNAi) response in insect pests. In this study, we applied a Weighted Gene Co-expression Network Analysis (WGCNA) to diet-based RNA-Seq data as a method to facilitate the discovery of novel target genes for pest control. <i>Nieman-Pick type c 1 homolog b</i> (<i>NPC1b</i>) was identified as the gene with the highest correlation values to hemolymph cholesterol levels and larval size. Functional characterization of the gene supported <i>CsNPC1b</i> expression with dietary cholesterol uptake and insect growth. This study revealed the critical role of NPC1b for intestinal cholesterol absorption in lepidopteran insects and highlights the utility of the WGCNA approach for identifying new pest management targets.
A critical role for CsCYP15C1 function in molting is supported by sequence similarity with known juvenile hormone epoxidases, its expression profile, and abnormal molting phenotypes associated with RNA-mediated knockdown. CsCYP15C1 is thus a prime target for controlling C. suppressalis. Furthermore, RNAi-mediated characterization of candidate gene function can be enhanced by incorporating an enveloping nanomaterial. © 2020 Society of Chemical Industry.
The striped rice stem borer, Chilo suppressalis Walker, is one of the most destructive rice pests in Asia. Insecticidal crystal proteins (Cry toxins) produced by Bacillus thuringiensis are widely used as biopesticides or in developing transgenic crops for pest management. In this study, we tested the involvement of two newly cloned C. suppressalis cadherins (CsCAD3 and CsCAD4) in the toxicity of Cry1Ab/Ac, Cry2Aa and Cry1Ca. Our results showed that CsCAD4 was expressed highest in the midgut, whereas CsCAD3 was expressed highest in the epidermis. The feeding of double-stranded RNA specific to CsCAD3 and CsCAD4 respectively significantly suppressed the expressions of target gene. The knockdown of CsCAD3 significantly reduced the mortality of larvae to Cry1Ab/Ac, whereas knockdown of CsCAD4 significantly decreased the larval susceptibility to Cry2Aa. In contrast, reduced expressions of CsCAD3 or CsCAD4 were not interacted with larval susceptibility to Cry1Ca. Our results suggest that CsCAD3 and CsCAD4 function in Cry toxin toxicity and these findings will help us to better understand the action mechanism of Cry toxins in C. suppressalis.
The study was carried out on cotton variety Giza 86 during two following seasons in 2017 and 2019 at the experimental farms of the faculties of Agriculture, Saba Bacha and El-Chatby, Alexandria, Egypt. The obtained results indicated that the highest efficacy was gained by Novatrin/ Radiant and Novatrin/ Super Mix, which decreased the incidence of Earias insulana (0.84 and 1.24 larvae /10 green bolls, in respect) and pectinophora gossypiella infestation and increased the cotton yield up to (57.8 Kent / fed(. Furthermore, Novatrin/ Super Mix and Super Mix/Radiant -achieved the lowest value of yield losses(9.6% and 10.1%, respectively) during the cotton season of 2017, in addition to the foliar treatments of Novatrin/ Super Mix/Radiant and Super Mix/ Potasin-F/ Radiant gave higher efficacy in reducing the level of the spiny bollworm infestation(0.53 and 0.65 larvae /10 green bolls, respectively) in 2019 season, while the application of Novatrin/ Super Mix/Radiant and Novatrin/Potasin-F/Radiant , increased the cotton yield (106.2% and 84.9%, in respect) resulting in decreasing yield losses during the 2019 cotton season, while the foliar application of Novatrin / Super Mix, increased cotton fiber length(34.21mm), while the application of lambda -cyhalothrin and Super Mix greatly increased fiber strength (50.36 and 50.16 gram/ tex, respectively), the highest increased value of fiber micronair reading was detected for the performed application of Super Mix / Radiant (4.88 millitex)during the 2017 cotton season, while the application of Novatrin / Potasin-F / Radiant increased fiber length (33.66 mm), Novatrin / Super Mix / Radiant treatments also increased fiber strength (45.53 gram/ tex) and the value of elongation (5.60%), as well as, application of Novatrin / Potasin-F/ chloropyrifos increased value of fiber micronair reading (4.88 millitex) during the 2019 cotton season.
I am seeking collaborations with researchers working in genomics, bioinformatics, AI for life sciences, and large-scale data analysis. I br…
I am currently working on a study to examine the effect of copper deficiency on the number of white blood cells, Neutrophils in particular,…